Match the following proteins with their respective role in regulation of phage lambda life cycle.Column – A Column – B 1. CI i. Establishes the lytic cycle 2. Cro ii. Controls expression of late genes 3. PN iii. Establishes and maintains lysogeny 4. PQ iv. Controls expression of delayed early genes
1 – iii, 2 – i, 3 – iv, 4 – ii
CI is the lambda repressor: it binds OR and OL, represses lytic promoters and activates its own synthesis from PRM, so it establishes and maintains lysogeny.
Cro binds the same operators with reverse affinity (OR3 first), shutting off PRM and committing the phage to the lytic cycle, so Cro establishes the lytic pathway.
The N protein (pN) is an antiterminator that allows transcription from PL and PR to read through terminators into the delayed early genes (including cII, cIII, O, P and Q).
The Q protein (pQ) is an antiterminator acting at the late promoter PR′, permitting transcription of the late genes for head, tail and lysis proteins.
Therefore the matches are CI–lysogeny, Cro–lytic cycle, N–delayed early genes and Q–late genes; the code pairing CI with the lytic cycle and Cro with lysogeny reverses their roles.
Hence, 1 – iii, 2 – i, 3 – iv, 4 – ii.
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.................... is a client server application that identifies each host on the internet with a unique user-friendly name.
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