The PCR technique that allows measuring of DNA amplification at each cycle of PCR rather than the end point detection is:
Real Time PCR
Real Time PCR, also called quantitative PCR (qPCR), couples DNA amplification with continuous fluorescence detection using intercalating dyes such as SYBR Green or sequence-specific probes such as TaqMan. The fluorescence signal is recorded at the end of every thermal cycle, so the amount of amplified product is monitored in real time and quantified from the threshold cycle value.
Hot start PCR only prevents non-specific priming before thermal cycling begins, nested PCR uses two successive primer pairs for specificity, and Reverse Transcriptase PCR first converts RNA to cDNA before amplification; none of these inherently measure product accumulation during every cycle.
Hence, the PCR technique that measures DNA amplification at each cycle is Real Time PCR.
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