In the enzymolysis, the following graph represent Lineweaver-Burk plots for :
Uncompetative inhibition
The stem carries a drawn graph; the four options are text and are reproduced in full above.
A Lineweaver-Burk plot graphs \(\frac{1}{v}\) against \(\frac{1}{[S]}\), giving a straight line with slope \(\frac{K_m}{V_{max}}\), y-intercept \(\frac{1}{V_{max}}\) and x-intercept \(-\frac{1}{K_m}\). Each type of inhibition changes these in its own signature way, so the pattern of lines identifies the mechanism.
Competitive inhibition — the inhibitor binds the free enzyme at the active site and competes with substrate. \(K_m\) rises but \(V_{max}\) is unchanged, because enough substrate still saturates the enzyme. The lines therefore intersect on the y-axis.
Non-competitive inhibition — the inhibitor binds equally well to E and to ES at a separate site. \(V_{max}\) falls but \(K_m\) is unchanged, so the lines intersect on the x-axis.
Uncompetitive inhibition — the inhibitor binds only to the enzyme-substrate complex, never to free enzyme. Both \(V_{max}\) and \(K_m\) fall, and crucially they fall by the same factor, so the ratio \(\frac{K_m}{V_{max}}\) — the slope — stays constant. The signature is therefore a set of parallel lines.
So the reading rule is simple: lines meeting on the y-axis mean competitive, lines meeting on the x-axis mean non-competitive, and parallel lines mean uncompetitive.
Per the official final answer key the answer is uncompetitive inhibition.
Similar Questions
Iron-enterobactin [Fe (ent)]3- complex has a dissociation content of 10-49 at pH = 7. Calculate the number of uncomplexed Fe3+ ions in a litre of 1 M solution in water at pH = 7.
The polypyrrole ring present in vitamin B12 is :
The metal ions that are commonly present at the active site of hydrolytic enzymes is :
Enzymolysis with the following mechanism :
\[ E + S \xrightleftharpoons[k_{-1}]{k_1} ES \] \[ ES \xrightarrow{k_2} E + P \] \[ E + I \rightleftharpoons EI \] \[ \therefore\ (I \equiv \text{inhibitor}) \quad I_1 < I_2 < I_3 \]
The following behaviors are noted.
Orders of the reaction, at point `A' and point `B' marked on the graph would be :
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