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Question

Identify the enzymes involved in DNA replication for their functions.

EnzymeFunction
a.DNA polymerase Ii.Unzipping the DNA helix
b.Primaseii.Supercoiling
c.Helicaseiii.Remove primer and close gaps
d.Gyraseiv.Synthesize RNA primer

This question was previously asked in
MH SET 2026 Paper 1 Question Paper (25-Oct-2026)
The correct answer is

a – iii, b – iv, c – i, d – ii

DNA polymerase I uses its 5′→3′ exonuclease to remove RNA primers of Okazaki fragments and its polymerase activity to fill the resulting gaps, so it matches primer removal and gap closing.

Primase (DnaG) synthesises the short RNA primers that provide the 3′-OH needed by DNA polymerase III; helicase (DnaB) unwinds the parental double helix at the fork.

DNA gyrase, a type II topoisomerase, introduces negative supercoils and relieves the positive supercoiling that builds up ahead of the fork.

Therefore the matches are polymerase I–primer removal, primase–RNA primer, helicase–unwinding and gyrase–supercoiling.

Hence, a – iii, b – iv, c – i, d – ii.

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