Match List I with List II Codes:List I List II a) Visualization of DNA in gel electrophoresis i) Chitinase b) Precipitation of DNA ii) Lysozyme c) Breaking of cell wall of bacteria iii) Chilled ethanol d) Breaking of cell wall of fungus iv) Ethidium bromide
a - iv, b - iii, c - ii, d - i
In recombinant DNA technology, isolating, purifying and analysing DNA needs a specific reagent at each step, as described in NCERT.
a) Visualization of DNA in gel electrophoresis: separated DNA fragments are not visible on their own; they are stained with ethidium bromide and seen as bright orange bands under UV light, so a matches iv.
b) Precipitation of DNA: after RNA and proteins are removed, purified DNA precipitates out on the addition of chilled ethanol and is seen as a collection of fine threads in the suspension, so b matches iii.
c) Breaking of cell wall of bacteria: the enzyme lysozyme digests the bacterial cell wall to release the DNA, so c matches ii.
d) Breaking of cell wall of fungus: the fungal cell wall contains chitin, which is broken down by chitinase, so d matches i.
The other code sets pair chilled ethanol with staining, chitinase with precipitation or bacterial walls, or lysozyme with precipitation, each of which contradicts at least one of these steps.
a - iv, b - iii, c - ii, d - i option is correct.
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