Given below are the sequence of events in the production of human insulin by Eli Lilly. a) Preparation of two DNA sequences corresponding to chain A and B of human insulin. b) Production of chain A and B separately. c) Introduction of chains in plasmids of E.coli. d) Extraction of chain A and B combining them by creating disulphide bonds. Choose the correct sequence of events.
(a), (c), (b), (d)
In 1983 Eli Lilly, an American company, prepared two DNA sequences corresponding to the A and B chains of human insulin and used recombinant DNA technology to make functional human insulin in bacteria.
Step 1 — The two DNA sequences coding for chain A and chain B are prepared (a).
Step 2 — These sequences are introduced into plasmids of E. coli, so the bacteria carry the insulin chain genes (c).
Step 3 — The transformed bacteria produce chain A and chain B separately (b).
Step 4 — Chains A and B are extracted and combined by creating disulphide bonds to form mature human insulin (d).
The chains cannot be produced before the genes are placed in the host plasmid, and they cannot be extracted and joined before they are produced, so any order putting (b) before (c) or (d) before (b) is not possible.
(a), (c), (b), (d) option is correct.
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