In November 2025, ICMR licensed indigenous diagnostic tools for the rapid detection of vector-borne diseases. Which technology primarily enables this single-tube simultaneous detection of Dengue, Chikungunya, and Zika?
Single-tube Multiplex Real-Time RT-PCR Method
All three diseases are caused by RNA viruses, so the test first reverse transcribes viral RNA into DNA and then amplifies it, which is what RT-PCR does.
The multiplex form of the assay puts primer and probe sets for dengue, chikungunya and Zika into the same reaction tube, each probe carrying a different fluorescent dye. The real-time machine reads the dyes on separate fluorescence channels, so one tube and one run tell the laboratory which of the three viruses is present.
A portable machine only changes where the test is run, not how three targets are read together, and the CRISPR based and AI based options are not the chemistry that makes the simultaneous single-tube detection possible.
Hence, the answer is Single-tube Multiplex Real-Time RT-PCR Method.
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