Actual site of dark reaction in photosynthesis inside cytoplast is
Stroma
The dark reaction takes place in the stroma of the chloroplast — option 3.
The structure of a chloroplast. It is a double-membrane organelle containing a system of flattened sacs and a fluid ground substance :
| Part | What it is | Which reaction |
|---|---|---|
| Thylakoid | A flattened membrane sac holding the chlorophyll | Light reaction — photolysis of water, ATP and NADPH made |
| Granum | A stack of thylakoids; grana lamellae are the membranes joining them | |
| Stroma | The colourless fluid matrix around the grana; holds enzymes, DNA and ribosomes | Dark reaction — the Calvin cycle |
Why the two stages are separated. The light reaction needs the pigments held in a membrane, so that the energy of an absorbed photon can be passed along an electron-transport chain and used to pump protons; a membrane is essential to it. The dark reaction is ordinary enzyme chemistry — the fixing of CO2 by RuBisCO and its reduction to sugar — and enzymes work in solution. So one stage is membrane-bound and the other is not.
What the dark reaction does. Carbon dioxide is attached to a five-carbon sugar, ribulose bisphosphate, and the product is reduced to carbohydrate using the ATP and NADPH made in the light reaction :
\(6\text{CO}_{2}+18\text{ATP}+12\text{NADPH}\rightarrow\text{C}_{6}\text{H}_{12}\text{O}_{6}+\ldots\)
A caution about the name. “Dark reaction” does not mean it happens at night or requires darkness — it means only that light is not directly involved. It stops soon after the light does, because it depends on the ATP and NADPH the light reaction supplies. For that reason it is better called the light-independent reaction or the Calvin cycle, after Melvin Calvin, who traced it with radioactive carbon-14 and received the Nobel Prize in 1961.
Hence, the answer is Stroma.
Which component of fertilizer is used for stimulating early growth purpose ?
In which organisms does spore formation occur as a mode of reproduction ?
Which of the following the chlorophyll in photosynthesis is used for ?
Buds are typically found
A nail is nailed into the trunk of a tree at a point 1 m from the soil level. After 2 years the nail will
Quinine is a common drug for malaria that is obtained from a plant. Which part of the plant yields this drug ?
Turmeric powder is obtained from which part of turmeric plant ?
Which of the following is a type of nutrition?
I. Heterotrophic
II. Autotrophic
Which of the following are the 5 groups that plants are divided into?
Fungi are always
In which of the following plants root is the food storage organ?
Which of the following does NOT form an essential part of the process of photosynthesis?