Cell Culture: Sodium Bicarbonate for pH Control
Sodium bicarbonate ($NaHCO_3$) is a standard component added to animal cell culture media primarily to regulate the pH.
Role in pH Maintenance
- Cell culture media require a stable pH, typically around 7.2 to 7.4, for optimal cell function and survival.
- Sodium bicarbonate acts as part of a bicarbonate buffer system, working in conjunction with dissolved carbon dioxide ($CO_2$) from the air or incubator atmosphere.
- This system neutralizes acids or bases produced during cell metabolism, preventing drastic pH shifts.
- A stable pH ensures proper enzyme activity and cellular processes.
Addressing Other Options
- Aeration: Gas exchange ($O_2$/$CO_2$) is managed through incubator settings and media surface area, not bicarbonate concentration.
- Iron Uptake: While iron is necessary, its availability and uptake are influenced by chelators and specific media composition, not primarily by bicarbonate.
- Cell Adhesion: Adherence to plastic surfaces is typically facilitated by the surface treatment of the culture dish and components like serum, not by sodium bicarbonate.
Similar Questions
- In Polymerase Chain Reaction (PCR) primers are used to provide:
- To detect specific proteins in a sample we use:
- The most commonly used stain for visualising/observing the DNA fragments is:
- The target sequence in a DNA can be determined/ identified in Southern blotting by:
- R$_f$ value depends on:
- Barr body is an example of:
Repeated sequence TTAGGC in humans is present at :
AATCCG- The attachment site for microtubules that separates chromosomes during cell division:
- The genetic material of a prokaryotic cell is present in:
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Important Questions from Mixed Topic (CUET PG)
- Who was the founder of Bolshevik Communist party?
- What is the key guide to statecraft in the realist tradition?
- Chronologically arrange the events in the Cold War period.
A. Berlin Wall is constructed
B. Communist China joins the UN
C. Soviet invasion of Czechoslovakia
D. Berlin Blockade
Choose the correct answer from the options given below: Morgenthau's principles of political realism are:
A. Politics is rooted in permanent and unchanging human nature which is basically self centred, self-regarding and self-interested
B. Politics is an autonomous sphere of action and cannot therefore be reduced to morals
C. International Politics is an arena of conflicting self-interests
D. The ethics of international relations is situational ethics which is very different from private morality
Choose the correct answer from the options given below:Who among the following political thinkers consider the anarchical self help system to be a compelling factor for States to maximise their relative power positions?
