PCR Primers Function: Providing the 3'-OH Group
Polymerase Chain Reaction (PCR) uses primers to initiate the synthesis of new DNA strands.
Primer Role in DNA Synthesis
- Primers bind to the target DNA template.
- DNA polymerase synthesizes the new strand.
- DNA polymerase requires a starting point with a free 3'-OH group.
Primers provide this required 3'-OH group at their 3' end. DNA polymerase uses this group to start adding nucleotides, extending the new DNA strand.
The 5' end and phosphate groups (like PO$_4^{ - }`) are not involved in initiating this process.
Final Answer: The correct option is 3' -OH, as it's the functional group provided by primers for DNA polymerase initiation.
Similar Questions
- To detect specific proteins in a sample we use:
- The most commonly used stain for visualising/observing the DNA fragments is:
- The target sequence in a DNA can be determined/ identified in Southern blotting by:
- R$_f$ value depends on:
- Barr body is an example of:
Repeated sequence TTAGGC in humans is present at :
AATCCG- The attachment site for microtubules that separates chromosomes during cell division:
- The genetic material of a prokaryotic cell is present in:
- During denaturation which bonds in protein structure are NOT broken:
- The degradation of stored glycogen in liver and muscles is known as:
Important Questions from Mixed Topic (CUET PG)
- Who was the founder of Bolshevik Communist party?
- What is the key guide to statecraft in the realist tradition?
- Chronologically arrange the events in the Cold War period.
A. Berlin Wall is constructed
B. Communist China joins the UN
C. Soviet invasion of Czechoslovakia
D. Berlin Blockade
Choose the correct answer from the options given below: Morgenthau's principles of political realism are:
A. Politics is rooted in permanent and unchanging human nature which is basically self centred, self-regarding and self-interested
B. Politics is an autonomous sphere of action and cannot therefore be reduced to morals
C. International Politics is an arena of conflicting self-interests
D. The ethics of international relations is situational ethics which is very different from private morality
Choose the correct answer from the options given below:Who among the following political thinkers consider the anarchical self help system to be a compelling factor for States to maximise their relative power positions?
