Question
In Polymerase Chain Reaction (PCR) primers are used to provide:
This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
3' -OH
PCR Primers Function: Providing the 3'-OH Group
Polymerase Chain Reaction (PCR) uses primers to initiate the synthesis of new DNA strands.
Primer Role in DNA Synthesis
- Primers bind to the target DNA template.
- DNA polymerase synthesizes the new strand.
- DNA polymerase requires a starting point with a free 3'-OH group.
Primers provide this required 3'-OH group at their 3' end. DNA polymerase uses this group to start adding nucleotides, extending the new DNA strand.
The 5' end and phosphate groups (like PO$_4^{ - }`) are not involved in initiating this process.
Final Answer: The correct option is 3' -OH, as it's the functional group provided by primers for DNA polymerase initiation.
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