DNA Visualization Stain Identification
The primary stain used for observing DNA fragments, especially after gel electrophoresis, is Ethidium Bromide (EtBr).
Why Ethidium Bromide?
- Ethidium Bromide is a fluorescent intercalating agent.
- It binds specifically to DNA and RNA molecules.
- When exposed to ultraviolet (UV) light, the EtBr-DNA complex fluoresces bright orange, making the DNA bands visible.
- This fluorescence allows for easy detection and imaging of DNA fragments separated by size.
Other Stains and Their Uses
- Coomassie Brilliant Blue: Primarily used to stain proteins, not nucleic acids.
- Safranin: Often used as a counterstain in plant histology and for staining bacterial cell walls.
- Crystal Violet: Commonly used to stain bacterial cell walls (Gram staining) and in histology.
Therefore, for the specific purpose of visualising DNA fragments, Ethidium Bromide is the standard and most common choice.
Similar Questions
- In Polymerase Chain Reaction (PCR) primers are used to provide:
- To detect specific proteins in a sample we use:
- The target sequence in a DNA can be determined/ identified in Southern blotting by:
- R$_f$ value depends on:
- Barr body is an example of:
Repeated sequence TTAGGC in humans is present at :
AATCCG- The attachment site for microtubules that separates chromosomes during cell division:
- The genetic material of a prokaryotic cell is present in:
- During denaturation which bonds in protein structure are NOT broken:
- The degradation of stored glycogen in liver and muscles is known as:
Important Questions from Mixed Topic (CUET PG)
- Who was the founder of Bolshevik Communist party?
- What is the key guide to statecraft in the realist tradition?
- Chronologically arrange the events in the Cold War period.
A. Berlin Wall is constructed
B. Communist China joins the UN
C. Soviet invasion of Czechoslovakia
D. Berlin Blockade
Choose the correct answer from the options given below: Morgenthau's principles of political realism are:
A. Politics is rooted in permanent and unchanging human nature which is basically self centred, self-regarding and self-interested
B. Politics is an autonomous sphere of action and cannot therefore be reduced to morals
C. International Politics is an arena of conflicting self-interests
D. The ethics of international relations is situational ethics which is very different from private morality
Choose the correct answer from the options given below:Who among the following political thinkers consider the anarchical self help system to be a compelling factor for States to maximise their relative power positions?
