Question
The most commonly used stain for visualising/observing the DNA fragments is:
This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
Ethidium Bromide
DNA Visualization Stain Identification
The primary stain used for observing DNA fragments, especially after gel electrophoresis, is Ethidium Bromide (EtBr).
Why Ethidium Bromide?
- Ethidium Bromide is a fluorescent intercalating agent.
- It binds specifically to DNA and RNA molecules.
- When exposed to ultraviolet (UV) light, the EtBr-DNA complex fluoresces bright orange, making the DNA bands visible.
- This fluorescence allows for easy detection and imaging of DNA fragments separated by size.
Other Stains and Their Uses
- Coomassie Brilliant Blue: Primarily used to stain proteins, not nucleic acids.
- Safranin: Often used as a counterstain in plant histology and for staining bacterial cell walls.
- Crystal Violet: Commonly used to stain bacterial cell walls (Gram staining) and in histology.
Therefore, for the specific purpose of visualising DNA fragments, Ethidium Bromide is the standard and most common choice.
Was this answer helpful?
Similar Questions
- Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: The amino acid sequences of Histones H$_3$ and H$_4$ are not highly conserved.
Reason R: Nearly all of the amino acids in a histone molecule are engaged in an interaction with either DNA or another histone.
In the light of the above statements, choose the most appropriate answer from the options given below - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: X chromosome is acrocentric and Y chromosome is submetacentric.
Reason R: This is based on the position of centromere on the chromosome.
In the light of the above statements, choose the most appropriate answer from the options given below - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Electron transport chain (ETC) and ATP synthesizing system are located on the inner mitochondrial membrane.
Reason R: The inner surface of the inner mitochondrial membrane possess phosphorylating subunits.
In the light of the above statements, choose the most appropriate answer from the options given below Match the LIST-I with LIST-II
LIST-I LIST-II A. Gas Chromatography I. Separation of ionic solutes B. Affinity Chromatography II. Separation based on size of molecules C. Gel-Filtration Chromatography III. Separation of biomolecules with different binding specificities D. Ion-Exchange Chromatography IV. Separation of volatile components - Select the correct sequence of morphological changes occurring in Apoptosis
A. Membrane blebbing
B. Phagocytosis
C. Nuclear fragmentation
D. Apopotic bodies
Choose the correct answer from the options given below: - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Bovine serum albumin (BSA) is not immunogenic when injected into cow but is strongly immunogenic when injected in rabbit.
Reason R: The greater the phylogenetic distance between two species, the less the structural disparity between them.
In the light of the above statements, choose the most appropriate answer from the options given below - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Father with mitochondrial gene defect cannot transmit the disease to his offspring.
Reason R: Mitochondria are the cell organelles which contain DNA.
In the light of the above statements, choose the most appropriate answer from the options given below - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Leaves exposed to the Sun, are thinner, have larger area of lamina have more chlorophyll and less stomata.
Reason R: Plants become structurally and physiologically adapted to the amount of solar radiations received.
In the light of the above statements, choose the most appropriate answer from the options given below - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Nervous and Endocrine Systems act together to coordinate functions of all body systems.
Reason R: Responses of the endocrine system are often slower but their influence is broader and they regulate all types of body cells.
In the light of the above statements, choose the most appropriate answer from the options given below - Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Cardiac excitation normally begins in the sinoatrial (SA) node.
Reason R: SA node cells do not have a stable resting potential. They depolarize to threshold spontaneously.
In the light of the above statements, choose the most appropriate answer from the options given below
Important Questions from Mixed Topic (CUET PG)
- Kalpsutra, the illustrated canonical text is from:-
- The Harappan city almost exclusively devoted to craft production was-:
- Mohandas Karamchand Gandhi launched quit India movement after the failure of:-
- The "Objectives Resolution" was introduced in constituent assembly by:-
- Who among the following was not a member of the constituent assembly:-
Need Expert Advice?
Upcoming Exams
GATE
February 06, 2027
Test Series
CUET
CUET PG Psychology (HUQP20) 2026 Mock Test Series
10 Tests
792 Attempts
3.7(15)
English
More Questions from CUET PG
