Question
The most commonly used stain for visualising/observing the DNA fragments is:
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CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
Ethidium Bromide
DNA Visualization Stain Identification
The primary stain used for observing DNA fragments, especially after gel electrophoresis, is Ethidium Bromide (EtBr).
Why Ethidium Bromide?
- Ethidium Bromide is a fluorescent intercalating agent.
- It binds specifically to DNA and RNA molecules.
- When exposed to ultraviolet (UV) light, the EtBr-DNA complex fluoresces bright orange, making the DNA bands visible.
- This fluorescence allows for easy detection and imaging of DNA fragments separated by size.
Other Stains and Their Uses
- Coomassie Brilliant Blue: Primarily used to stain proteins, not nucleic acids.
- Safranin: Often used as a counterstain in plant histology and for staining bacterial cell walls.
- Crystal Violet: Commonly used to stain bacterial cell walls (Gram staining) and in histology.
Therefore, for the specific purpose of visualising DNA fragments, Ethidium Bromide is the standard and most common choice.
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