Question
To detect specific proteins in a sample we use:
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CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
Western Blotting
Detecting Specific Proteins via Blotting
The primary method used to detect specific proteins within a biological sample is Western Blotting. This technique is also known as immunoblotting.
Understanding Blotting Techniques
- Western Blotting: Specifically designed to identify and detect specific proteins. It uses antibodies to bind to the target protein after the proteins have been separated by size (usually via electrophoresis) and transferred to a membrane.
- Northern Blotting: Used for detecting specific sequences of RNA, not proteins.
- Eastern Blotting: While it also detects proteins, it's typically used to detect post-translational modifications like glycosylation, and is less common than Western Blotting for general protein detection.
- Footprinting: This technique is used to study protein-DNA interactions by identifying the regions of DNA bound by proteins.
Therefore, Western Blotting is the standard and most appropriate technique among the choices for detecting specific proteins.
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