Detecting Specific Proteins via Blotting
The primary method used to detect specific proteins within a biological sample is Western Blotting. This technique is also known as immunoblotting.
Understanding Blotting Techniques
- Western Blotting: Specifically designed to identify and detect specific proteins. It uses antibodies to bind to the target protein after the proteins have been separated by size (usually via electrophoresis) and transferred to a membrane.
- Northern Blotting: Used for detecting specific sequences of RNA, not proteins.
- Eastern Blotting: While it also detects proteins, it's typically used to detect post-translational modifications like glycosylation, and is less common than Western Blotting for general protein detection.
- Footprinting: This technique is used to study protein-DNA interactions by identifying the regions of DNA bound by proteins.
Therefore, Western Blotting is the standard and most appropriate technique among the choices for detecting specific proteins.
Similar Questions
- In Polymerase Chain Reaction (PCR) primers are used to provide:
- The most commonly used stain for visualising/observing the DNA fragments is:
- The target sequence in a DNA can be determined/ identified in Southern blotting by:
- R$_f$ value depends on:
- Barr body is an example of:
Repeated sequence TTAGGC in humans is present at :
AATCCG- The attachment site for microtubules that separates chromosomes during cell division:
- The genetic material of a prokaryotic cell is present in:
- During denaturation which bonds in protein structure are NOT broken:
- The degradation of stored glycogen in liver and muscles is known as:
Important Questions from Mixed Topic (CUET PG)
- Who was the founder of Bolshevik Communist party?
- What is the key guide to statecraft in the realist tradition?
- Chronologically arrange the events in the Cold War period.
A. Berlin Wall is constructed
B. Communist China joins the UN
C. Soviet invasion of Czechoslovakia
D. Berlin Blockade
Choose the correct answer from the options given below: Morgenthau's principles of political realism are:
A. Politics is rooted in permanent and unchanging human nature which is basically self centred, self-regarding and self-interested
B. Politics is an autonomous sphere of action and cannot therefore be reduced to morals
C. International Politics is an arena of conflicting self-interests
D. The ethics of international relations is situational ethics which is very different from private morality
Choose the correct answer from the options given below:Who among the following political thinkers consider the anarchical self help system to be a compelling factor for States to maximise their relative power positions?
