Choose the most suitable option to replace the highlighted part of the sentence: Despite of his persistent efforts, the proposal was rejected.
Despite his persistence
'Despite' is a preposition and is used directly before a noun or noun phrase, without 'of': 'despite his efforts'. 'Despite of' is a common error.
Its equivalent 'in spite' always needs 'of': 'in spite of his persistence'. So 'In spite his persistence' is wrong.
'Though' and 'although' are conjunctions that introduce a clause with a verb, so 'Though of his persistence' and 'Although his persistent' are ungrammatical.
'Despite his persistence' correctly uses the preposition with a noun phrase and keeps the meaning of the original phrase.
Hence, the answer is Despite his persistence: Despite his persistence, the proposal was rejected.
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