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Question

Which enzyme in meat is responsible for increase in tenderness during aging ?

This question was previously asked in
MH SET 2025 Paper 2 Sociology Question Paper (15-Jun-2025)
The correct answer is

Cathepsins

Cathepsins option is correct.

Post-mortem tenderisation of meat during aging is driven by endogenous proteolytic enzymes that break down the myofibrillar and connective tissue proteins responsible for toughness. The two enzyme systems responsible are the calcium-activated calpains and the lysosomal cathepsins.

Cathepsins (cathepsin B, D, L and H) are aspartic and cysteine proteases released from lysosomes during the pH drop after slaughter; they hydrolyse actin, myosin, titin and collagen, softening the muscle fibre matrix and increasing tenderness during the aging period.

Lipases act on triglycerides (flavour development, not tenderness), amylases hydrolyse starch and are irrelevant in muscle tissue, and lysozymes break bacterial peptidoglycan and play no role in meat tenderisation.

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