Question
Match the LIST-I with LIST-IILIST-I LIST-II A. Gas Chromatography I. Separation of ionic solutes B. Affinity Chromatography II. Separation based on size of molecules C. Gel-Filtration Chromatography III. Separation of biomolecules with different binding specificities D. Ion-Exchange Chromatography IV. Separation of volatile components
This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
A-IV, B-III, C-II, D-I
Chromatography Separation Principles Explained
This question requires matching specific chromatography techniques (LIST-I) with their fundamental separation principles (LIST-II).
Matching Chromatography Techniques
The correct matching is determined by the unique mechanism each chromatography type uses for separation:
- A. Gas Chromatography (GC): Separates volatile compounds based on their boiling points and interaction with a stationary phase.
- B. Affinity Chromatography: Exploits specific reversible binding interactions between an analyte and a ligand immobilized on the stationary phase.
- C. Gel-Filtration Chromatography (Size Exclusion Chromatography): Separates molecules based on their size and shape (hydrodynamic volume).
- D. Ion-Exchange Chromatography: Separates ionic compounds based on their charge and the charge of the stationary phase.
Matching Summary Table
| LIST-I Technique | LIST-II Principle | Match |
|---|---|---|
| A. Gas Chromatography | IV. Separation of volatile components | A-IV |
| B. Affinity Chromatography | III. Separation of biomolecules with different binding specificities | B-III |
| C. Gel-Filtration Chromatography | II. Separation based on size of molecules | C-II |
| D. Ion-Exchange Chromatography | I. Separation of ionic solutes | D-I |
Conclusion
Based on the principles of each technique, the correct pairing is A-IV, B-III, C-II, D-I. This corresponds to Option 3, labelled as C in the provided answer.
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