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Question

Choose the correct sequence of enzymes involved in the preparatory phase of glycolysis:
A. Hexokinase
B. Phospho-fructo kinase I
C. Phospho-hexose isomerase
D. Aldolase
E. Triose-phosphate Isomerase
Choose the correct answer from the options given below:

This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
A, C, B, D and E

Glycolysis Preparatory Phase Enzyme Sequence

The preparatory phase of glycolysis involves the initial steps where glucose is broken down into two three-carbon molecules. This phase requires energy input (ATP) and includes several enzymatic reactions.

Enzymes in Glycolysis Preparatory Phase

The sequence of key enzymes in the preparatory phase of glycolysis, starting from glucose, is as follows:

  1. Glucose is converted to Glucose-6-phosphate by Hexokinase (A).
  2. Glucose-6-phosphate is isomerized to Fructose-6-phosphate by Phospho-hexose isomerase (C).
  3. Fructose-6-phosphate is converted to Fructose-1,6-bisphosphate by Phospho-fructo kinase (B).
  4. Fructose-1,6-bisphosphate is cleaved into Dihydroxyacetone phosphate and Glyceraldehyde-3-phosphate by Aldolase (D).
  5. Dihydroxyacetone phosphate is isomerized to Glyceraldehyde-3-phosphate by Triose-phosphate Isomerase (E).

Correct Enzyme Sequence

Therefore, the correct sequence of enzymes involved in the preparatory phase of glycolysis is:

A (Hexokinase), C (Phospho-hexose isomerase), B (Phospho-fructo kinase), D (Aldolase), and E (Triose-phosphate Isomerase).

This sequence corresponds to the options provided.

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