A. Introduction of recombinant molecule into new host that expresses the gene
B. Use of restriction enzymes or PCR to generate fragments of DNA
C. Protein purification
D. Isolation of DNA to be cloned
E. Generation of a recombinant molecule by inserting DNA fragments into vectors
Choose the correct answer from the options given below :
Gene Cloning and Expression: Correct Step Order
To clone and express a gene, specific steps must be performed sequentially. Here is the breakdown:
Step 1: DNA Isolation
The process begins with the isolation of DNA containing the gene of interest. This involves extracting the genetic material from the source organism.
Step 2: DNA Fragmentation
Next, the isolated DNA is processed to obtain the specific gene fragment. This is achieved using tools like restriction enzymes or the Polymerase Chain Reaction (PCR) to cut or amplify the desired DNA segment.
Step 3: Recombinant Molecule Generation
The generated DNA fragment is then inserted into a suitable carrier molecule, known as a vector (e.g., a plasmid). This creates a recombinant molecule, combining the vector DNA and the gene of interest.
Step 4: Host Transformation
The recombinant molecule is introduced into a new host cell, typically a bacterium. This process is called transformation. The host cell then replicates, carrying the recombinant DNA.
Step 5: Protein Purification
If the goal is to produce the protein encoded by the cloned gene, the final step involves isolating and purifying the protein from the host cells after expression.
Final Sequence
Following these logical steps, the correct order is:
- D. Isolation of DNA to be cloned
- B. Use of restriction enzymes or PCR to generate fragments of DNA
- E. Generation of a recombinant molecule by inserting DNA fragments into vectors
- A. Introduction of recombinant molecule into new host that expresses the gene
- C. Protein purification
Therefore, the correct sequence is D, B, E, A, C.
Similar Questions
- Arrange the following steps of crossing over in correct order :
A. Pachytene stage
B. Synapsis of homologous chromosomes
C. Separation of homologous chromosomes
D. Breakage and reunion of chromatids
E. Formation of chiasmata
Choose the correct answer from the options given below : - Arrange the following steps in lac operon induction in correct order :
A. Formation of mRNA
B. Binding of lactose to repressor
C. RNA polymerase binds to promoter
D. Transcription of structural genes
E. Removal of repressor from operator
Choose the correct answer from the options given below : - Arrange the steps in southern blotting in correct order :
A. Transfer of DNA to nitrocellulose membrane
B. Detection of hybridized probe
C. Restriction digestion of DNA
D. Hybridization with labelled probe
E. Gel electrophoresis
Choose the correct answer from the options given below : - Arrange the steps of endospore formation in correct order :
A. Cortex and spore coat formation
B. Maturation and release of endospore
C. DNA replication and axial filament formation
D. Engulfment of forespore
E. Asymmetric septum formation
Choose the correct answer from the options given below : - Arrange the steps in antigen processing by endocytic pathway in correct order :
A. Recognition by CD4+ T cells
B. Antigen uptake by APC
C. Transport of MHC-peptide complex to cell surface
D. Peptide loading onto MHC class II molecule
E. Fusion of phagosome with lysosome
Choose the correct answer from the options given below : - Arrange the steps of agarose gel electrophoresis in correct order :
A. Preparation of agarose gel
B. Visualization under UV light
C. Loading of DNA samples
D. Migration of DNA fragments
E. Application of electric current
Choose the correct answer from the options given below : - Arrange the following steps of root nodule formation in correct order :
A. Formation of bacteroids and differentiation into nitrogen fixing symbiosomes
B. Attachment of bacterial cells to root hairs
C. Plant root hair with attached Rhizobium undergoes curling
D. Bacterial penetration into root hair cell and cell to cell spread of rhizobia
E. Plant root releases flavonoids
Choose the correct answer from the options given below : - Arrange the steps of life cycle of phage T4 in correct sequence.
A. Lysis of the host cell
B. Injection of phage DNA
C. Synthesis and assembly of phage components
D. Adsorption of the phage
E. Degradation of host DNA
Choose the correct answer from the options given below : - Arrange the following centrifugal speeds in an order to be used when a cell homogenate is to be separated into various fractions.
A. 1000 g
B. 80,000 g
C. 1,50,000 g
D. 20,000 g
E. 60,000 g
Choose the correct answer from the options given below : - Arrange the components of photosynthetic machinery of purple bacteria in a sequence followed for flow of electrons :
A. Quinone
B. P870
C. Cyt bC1 complex
D. Cyt C2
E. Pheophytin
Choose the correct answer from the options given below :
Important Questions from Mixed Topic (CUET PG)
- Who was the founder of Bolshevik Communist party?
- What is the key guide to statecraft in the realist tradition?
- Chronologically arrange the events in the Cold War period.
A. Berlin Wall is constructed
B. Communist China joins the UN
C. Soviet invasion of Czechoslovakia
D. Berlin Blockade
Choose the correct answer from the options given below: Morgenthau's principles of political realism are:
A. Politics is rooted in permanent and unchanging human nature which is basically self centred, self-regarding and self-interested
B. Politics is an autonomous sphere of action and cannot therefore be reduced to morals
C. International Politics is an arena of conflicting self-interests
D. The ethics of international relations is situational ethics which is very different from private morality
Choose the correct answer from the options given below:Who among the following political thinkers consider the anarchical self help system to be a compelling factor for States to maximise their relative power positions?
