A. 1000 g
B. 80,000 g
C. 1,50,000 g
D. 20,000 g
E. 60,000 g
Choose the correct answer from the options given below :
Cell Fractionation Order by Centrifugal Speed
Differential centrifugation is a technique used to separate cellular components based on their size and density. This separation is achieved by centrifuging a cell homogenate at progressively higher centrifugal forces (measured in 'g', representing Relative Centrifugal Force).
Understanding Differential Centrifugation
- Lower centrifugal speeds pellet larger and denser components first.
- As the speed increases, smaller and less dense components are pelleted.
- The process starts with the lowest speed and moves towards the highest speed.
Arranging Centrifugal Speeds
The centrifugal speeds provided are:
- A. 1000 g
- B. 80,000 g
- C. 1,50,000 g
- D. 20,000 g
- E. 60,000 g
To separate cell homogenate into various fractions, these speeds must be arranged in ascending order:
- 1000 g (Option A): This is the lowest speed, used to pellet the largest and densest components like nuclei.
- 20,000 g (Option D): The next higher speed, pelleting components such as mitochondria.
- 60,000 g (Option E): Used for pelleting smaller organelles like lysosomes and peroxisomes.
- 80,000 g (Option B): Further fractionation, potentially pelleting microsomes.
- 1,50,000 g (Option C): The highest speed, used to pellet the smallest components like ribosomes and soluble proteins.
Correct Sequence Determination
The correct order of centrifugal speeds for cell fractionation is therefore A, D, E, B, C.
This sequence matches Option 1.
Similar Questions
- Arrange the following steps of crossing over in correct order :
A. Pachytene stage
B. Synapsis of homologous chromosomes
C. Separation of homologous chromosomes
D. Breakage and reunion of chromatids
E. Formation of chiasmata
Choose the correct answer from the options given below : - Arrange the following steps in lac operon induction in correct order :
A. Formation of mRNA
B. Binding of lactose to repressor
C. RNA polymerase binds to promoter
D. Transcription of structural genes
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Choose the correct answer from the options given below : - Arrange the steps in southern blotting in correct order :
A. Transfer of DNA to nitrocellulose membrane
B. Detection of hybridized probe
C. Restriction digestion of DNA
D. Hybridization with labelled probe
E. Gel electrophoresis
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A. Cortex and spore coat formation
B. Maturation and release of endospore
C. DNA replication and axial filament formation
D. Engulfment of forespore
E. Asymmetric septum formation
Choose the correct answer from the options given below : - Arrange the steps in antigen processing by endocytic pathway in correct order :
A. Recognition by CD4+ T cells
B. Antigen uptake by APC
C. Transport of MHC-peptide complex to cell surface
D. Peptide loading onto MHC class II molecule
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Choose the correct answer from the options given below : - Arrange the steps of agarose gel electrophoresis in correct order :
A. Preparation of agarose gel
B. Visualization under UV light
C. Loading of DNA samples
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A. Formation of bacteroids and differentiation into nitrogen fixing symbiosomes
B. Attachment of bacterial cells to root hairs
C. Plant root hair with attached Rhizobium undergoes curling
D. Bacterial penetration into root hair cell and cell to cell spread of rhizobia
E. Plant root releases flavonoids
Choose the correct answer from the options given below : - Arrange the steps of life cycle of phage T4 in correct sequence.
A. Lysis of the host cell
B. Injection of phage DNA
C. Synthesis and assembly of phage components
D. Adsorption of the phage
E. Degradation of host DNA
Choose the correct answer from the options given below : - Arrange the components of photosynthetic machinery of purple bacteria in a sequence followed for flow of electrons :
A. Quinone
B. P870
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Choose the correct answer from the options given below : - Arrange the steps in cloning and expressing a gene in correct order :
A. Introduction of recombinant molecule into new host that expresses the gene
B. Use of restriction enzymes or PCR to generate fragments of DNA
C. Protein purification
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E. Generation of a recombinant molecule by inserting DNA fragments into vectors
Choose the correct answer from the options given below :
Important Questions from Mixed Topic (CUET PG)
- Who was the founder of Bolshevik Communist party?
- What is the key guide to statecraft in the realist tradition?
- Chronologically arrange the events in the Cold War period.
A. Berlin Wall is constructed
B. Communist China joins the UN
C. Soviet invasion of Czechoslovakia
D. Berlin Blockade
Choose the correct answer from the options given below: Morgenthau's principles of political realism are:
A. Politics is rooted in permanent and unchanging human nature which is basically self centred, self-regarding and self-interested
B. Politics is an autonomous sphere of action and cannot therefore be reduced to morals
C. International Politics is an arena of conflicting self-interests
D. The ethics of international relations is situational ethics which is very different from private morality
Choose the correct answer from the options given below:Who among the following political thinkers consider the anarchical self help system to be a compelling factor for States to maximise their relative power positions?
