A. Cortex and spore coat formation
B. Maturation and release of endospore
C. DNA replication and axial filament formation
D. Engulfment of forespore
E. Asymmetric septum formation
Choose the correct answer from the options given below :
The formation of bacterial endospores (sporulation) is a complex process involving several distinct stages. Arranging these stages correctly is crucial for understanding bacterial survival mechanisms.
The correct order of the steps for endospore formation is: C, E, D, A, B.
The process begins with the replication of the bacterial DNA. The replicated chromosomes migrate to opposite poles of the cell, forming axial filaments.
An asymmetric septum (a dividing wall) forms within the cell, separating the replicated DNA and a small amount of cytoplasm to form the pre-spore (forespore).
The larger mother cell membrane then engulfs the forespore, enclosing it within the cell.
Peptidoglycan layers form between the two membranes, creating the cortex. Protective layers known as the spore coat develop around the cortex.
The endospore fully matures, becoming resistant to environmental stress. Finally, the mother cell lyses (breaks down), releasing the free endospore.
By following the biological sequence of events, the correct order is determined to be C → E → D → A → B, which corresponds to Option D.