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Question

Arrange the steps of endospore formation in correct order :
A. Cortex and spore coat formation
B. Maturation and release of endospore
C. DNA replication and axial filament formation
D. Engulfment of forespore
E. Asymmetric septum formation
Choose the correct answer from the options given below :

This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
C, E, D, A, B

Endospore Formation Steps Explained

The formation of bacterial endospores (sporulation) is a complex process involving several distinct stages. Arranging these stages correctly is crucial for understanding bacterial survival mechanisms.

Correct Sequence of Endospore Formation

The correct order of the steps for endospore formation is: C, E, D, A, B.

  1. Step C: DNA replication and axial filament formation

    The process begins with the replication of the bacterial DNA. The replicated chromosomes migrate to opposite poles of the cell, forming axial filaments.

  2. Step E: Asymmetric septum formation

    An asymmetric septum (a dividing wall) forms within the cell, separating the replicated DNA and a small amount of cytoplasm to form the pre-spore (forespore).

  3. Step D: Engulfment of forespore

    The larger mother cell membrane then engulfs the forespore, enclosing it within the cell.

  4. Step A: Cortex and spore coat formation

    Peptidoglycan layers form between the two membranes, creating the cortex. Protective layers known as the spore coat develop around the cortex.

  5. Step B: Maturation and release of endospore

    The endospore fully matures, becoming resistant to environmental stress. Finally, the mother cell lyses (breaks down), releasing the free endospore.

Final Answer Derivation

By following the biological sequence of events, the correct order is determined to be C → E → D → A → B, which corresponds to Option D.

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