Assertion (A) : A cDNA library contains only expressed genes.
Reason (R) : A cDNA library is made from the mRNA molecules of a cell population.
In the light of the above statements, choose the most appropriate answer from the options given below :
Assertion (A) Analysis: cDNA Library Content
A cDNA (complementary DNA) library is created using DNA synthesized from messenger RNA (mRNA) templates.
- mRNA molecules are transcribed only from genes that are actively being expressed (transcribed) in a cell at a given time.
- Therefore, a cDNA library, being derived from mRNA, inherently represents the set of actively expressed genes within that cell population.
Assertion (A) is correct.
Reason (R) Analysis: cDNA Library Construction
The process of creating a cDNA library involves using the enzyme reverse transcriptase to synthesize DNA from isolated mRNA molecules.
- The starting material for cDNA synthesis is specifically the mRNA population present in the cells.
- This mRNA population reflects the genes that were being transcribed.
Reason (R) is correct.
Relationship Between Assertion (A) and Reason (R)
Reason (R) states that a cDNA library is made from mRNA. Since mRNA molecules are direct products of gene expression, the material used to build the library (mRNA) directly determines its content (expressed genes).
Therefore, the fact that a cDNA library is made from mRNA molecules (Reason R) is the direct reason why it contains only expressed genes (Assertion A).
Reason (R) provides the correct explanation for Assertion (A).
Similar Questions
- Blue-white screening is based on the activity of :
- Which of the following is not a feature of RNA ?
- RNAi mediated RNA interference mediates which of the following ?
- Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Some microorganisms can use nitrate as a terminal electron acceptor under anaerobic conditions.
Reason (R) : Nitrate can be fully reduced to ammonia in the process of denitrification.
In the light of the above statements, choose the most appropriate answer from the options given below : - Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Electrophoretic procedures are generally not used to purify proteins.
Reason (R) : Electrophoresis is an analytical method.
In the light of the above statements, choose the most appropriate answer from the options given below : - Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : E.coli is widely used as a host in RDT.
Reason (R) : E.coli grows rapidly and its genetics are well understood.
In the light of the above statements, choose the most appropriate answer from the options given below : - Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Biodiversity is normally greater in the lower latitudes as compared to the higher latitudes.
Reason (R) : Along the mountain gradients, biodiversity is normally greater in the lower altitudes as compared to the higher altitudes.
In the light of the above statements, choose the most appropriate answer from the options given below : - Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Ruminants are able to digest complex plant material.
Reason (R) : The Rumen community contains a community of microorganisms producing a variety of enzymes that can degrade plant polymers.
In the light of the above statements, choose the most appropriate answer from the options given below : - Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : IgG is the first immunoglobulin produced during a primary immune response.
Reason (R) : IgM has a pentameric structure that allows efficient activation of the complement system.
In the light of the above statements, choose the most appropriate answer from the options given below : - Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : The closer two loci reside along the axis of the chromosome, the more likely they are to be inherited together.
Reason (R) : The farther apart two linked loci, the more likely a random crossover event will occur in between them.
In the light of the above statements, choose the most appropriate answer from the options given below :
Important Questions from Mixed Topic (CUET PG)
- Kalpsutra, the illustrated canonical text is from:-
- The Harappan city almost exclusively devoted to craft production was-:
- Mohandas Karamchand Gandhi launched quit India movement after the failure of:-
- The "Objectives Resolution" was introduced in constituent assembly by:-
- Who among the following was not a member of the constituent assembly:-
