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Question

Arrange the following steps of root nodule formation in correct order :
A. Formation of bacteroids and differentiation into nitrogen fixing symbiosomes
B. Attachment of bacterial cells to root hairs
C. Plant root hair with attached Rhizobium undergoes curling
D. Bacterial penetration into root hair cell and cell to cell spread of rhizobia
E. Plant root releases flavonoids
Choose the correct answer from the options given below :

This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
E, B, C, D, A

Root Nodule Formation: Key Steps

The formation of root nodules is a complex symbiotic interaction essential for nitrogen fixation in legumes. The process involves sequential steps starting with chemical signaling and ending with functional nitrogen-fixing units.

Sequence of Events

Here are the steps involved in root nodule formation, arranged in the correct chronological order:

  1. E. Flavonoid Release: The process begins when the legume root releases specific flavonoids into the soil. These chemicals act as signals to attract and stimulate compatible Rhizobium bacteria.
  2. B. Bacterial Attachment: Responding to the flavonoid signal, Rhizobium bacteria attach themselves to the surface of the plant root hairs.
  3. C. Root Hair Curling: Upon attachment, the root hair undergoes significant curling, trapping the bacteria and preparing for entry.
  4. D. Bacterial Penetration: The bacteria penetrate the root hair cell wall, often through an 'infection thread' – a tube-like structure formed by the plant cell membrane. The bacteria then multiply and spread within the root cortex, moving cell-to-cell.
  5. A. Symbiosome Formation: Inside the root cells, the bacteria are released from the infection thread and differentiate into specialized nitrogen-fixing forms called bacteroids. These bacteroids reside within plant-derived membrane sacs, forming structures called symbiosomes, which are the sites of nitrogen fixation.

Correct Order Summary

Therefore, the correct order of the steps is:

E → B → C → D → A

This sequence highlights the progression from initial molecular signaling to the establishment of a functional nitrogen-fixing symbiosis within the root nodule.

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