An inverted repeat (IR) is a sequence of DNA that reads the same forwards on one strand as it does backwards on the complementary strand. This means the sequence is identical to its reverse complement. A common form is $S...RC(S)$, where $RC(S)$ is the reverse complement of $S$. A sequence that is its own reverse complement is called a palindrome.
We examine the given DNA sequences to identify which one contains an inverted repeat.
Let's analyze Option B: $ATGAGCCGGCTCTA TACTCGGCCGAGAT$.
The complete sequence is:
$ATGAGCCGGCTCTATACTCGGCCGAGAT$
This sequence contains the subsequence $CCGG$ appearing twice:
The subsequence $CCGG$ is a palindrome, meaning it is its own reverse complement ($RC(CCGG) = CCGG$). The presence of this palindromic sequence repeated within the larger DNA strand constitutes an inverted repeat structure ($CCGG...CCGG$).
Therefore, Option B carries an inverted repeat.
Consider the following multiple sequence alignment of four DNA sequences.
$\begin{matrix}A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T \end{matrix} $
Shannon's entropy of the above alignment is ________.
The transcription factor X binds a 10 base pair DNA stretch. In the DNA of an organism, X was found to bind at 20 distinct sites. An analysis of these 20 binding sites showed the following distribution:
| Base | Position in the binding site | |||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
| A | 11 | 0 | 0 | 0 | 16 | 2 | 4 | 0 | 4 | 3 |
| T | 3 | 0 | 19 | 0 | 1 | 3 | 4 | 20 | 2 | 4 |
| G | 4 | 20 | 0 | 0 | 2 | 4 | 6 | 0 | 12 | 2 |
| C | 2 | 0 | 1 | 20 | 1 | 11 | 6 | 0 | 2 | 11 |
What is the consensus sequence for the binding site of X?