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Question

Which one of the following DNA sequences carries an invert repeat?

The correct answer is
ATGAGCCGGCTCTA TACTCGGCCGAGAT

DNA Invert Repeat Definition

An inverted repeat (IR) is a sequence of DNA that reads the same forwards on one strand as it does backwards on the complementary strand. This means the sequence is identical to its reverse complement. A common form is $S...RC(S)$, where $RC(S)$ is the reverse complement of $S$. A sequence that is its own reverse complement is called a palindrome.

Sequence Analysis for Invert Repeats

We examine the given DNA sequences to identify which one contains an inverted repeat.

Option B: Identifying the Invert Repeat

Let's analyze Option B: $ATGAGCCGGCTCTA TACTCGGCCGAGAT$.

The complete sequence is:

$ATGAGCCGGCTCTATACTCGGCCGAGAT$

This sequence contains the subsequence $CCGG$ appearing twice:

  • The first instance of $CCGG$ starts at position 7.
  • The second instance of $CCGG$ starts at position 20 (using 1-based indexing).

The subsequence $CCGG$ is a palindrome, meaning it is its own reverse complement ($RC(CCGG) = CCGG$). The presence of this palindromic sequence repeated within the larger DNA strand constitutes an inverted repeat structure ($CCGG...CCGG$).

Therefore, Option B carries an inverted repeat.

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Important Questions from Molecular Structure of Genes and Chromosomes

  1. Consider the following multiple sequence alignment of four DNA sequences.

    $\begin{matrix}A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T  \end{matrix} $

    Shannon's entropy of the above alignment is ________.

  2. All pseudogenes DO NOT code for a __________.
  3. C-value paradox refers to
  4. The transcription factor X binds a 10 base pair DNA stretch. In the DNA of an organism, X was found to bind at 20 distinct sites. An analysis of these 20 binding sites showed the following distribution:

    Base  Position in the binding site
    12345678910
    A110001624043
    T301901342024
    G420002460122
    C2012011160211

    What is the consensus sequence for the binding site of X?

  5. Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?
    P. A larger E-value indicates higher sequence similarity
    Q. E-value < $10^{-10}$ indicates sequence homology
    R. A higher BLAST score indicates higher sequence similarity
    S. E-value > $10^{10}$ indicates sequence homology
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