Question
DNA sample collected from an unidentified bacterial species (Y) contains 13% of adenine. The G+C content (in percentage) of Y is ________
Understanding DNA Base Composition
This question involves calculating the Guanine (G) and Cytosine (C) content in a DNA sample based on the percentage of Adenine (A) provided.
Applying Chargaff's Rules
Chargaff's rules are essential here. They state that in a double-stranded DNA molecule:
- The amount of Adenine (A) is equal to the amount of Thymine (T).
- The amount of Guanine (G) is equal to the amount of Cytosine (C).
The total percentage of all bases (A, T, G, C) must equal 100%.
Calculation Steps
- Given Adenine Content: The DNA sample contains 13% Adenine (A).
$A = 13\%$ - Determine Thymine Content: According to Chargaff's rules, $A = T$.
Therefore, Thymine (T) content is also 13%.
$T = 13\%$ - Calculate Combined A+T Content: Add the percentages of Adenine and Thymine.
$A + T = 13\% + 13\% = 26\%$ - Calculate Combined G+C Content: The total DNA composition is 100%. Subtract the combined A+T content from 100% to find the G+C content.
$G + C = 100\% - (A + T)$
$G + C = 100\% - 26\%$
$G + C = 74\%$
Conclusion
The G+C content of the bacterial DNA sample (Y) is 74%.
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