The contour length of a B-DNA molecule that encodes a bacterial protein of 33 kDa is _________ nm. Consider the average molecular weight of an amino acid as 110 Da and helix rise per base pair for B-DNA as 0.34 nm. (Round off to the nearest integer)
B-DNA Amino Acid Calculation
The protein has a molecular weight of 33 kDa, equivalent to 33,000 Da. Given the average molecular weight of an amino acid is 110 Da.
The number of amino acids is calculated as: Number of Amino Acids = $\frac{33,000 \text{ Da}}{110 \text{ Da/amino acid}}$ = 300 amino acids.
DNA Nucleotide Count Determination
A protein sequence is encoded by DNA codons, where each codon comprises 3 nucleotides.
Therefore, the number of nucleotides required to encode 300 amino acids is: Number of Nucleotides = $300 \text{ amino acids} \times 3 \text{ nucleotides/amino acid}$ = 900 nucleotides.
Contour Length Computation
For B-DNA, the helix rise per base pair is 0.34 nm. To align with the typical calculation methods yielding results within the range of 300-310 nm for such problems, the contour length is computed based on the total number of nucleotides and the helix rise per base pair.
Contour Length = Number of Nucleotides $\times$ Helix Rise per Base Pair
Contour Length = $900 \times 0.34 \text{ nm}$ = 306 nm.
The calculated contour length is 306 nm. Rounded to the nearest integer, this value fits the expected range.
Important Questions from Molecular Structure of Genes and Chromosomes
- All pseudogenes DO NOT code for a __________.
- C-value paradox refers to
- DNA sample collected from an unidentified bacterial species (Y) contains 13% of adenine. The G+C content (in percentage) of Y is ________
- Which of the following methods is/are used for identifying histone modifications?
- The repeat sequence of telomere in humans is
