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Question

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : UV spectrophotometry is commonly used to estimate nucleic acids.
Reason (R) : Nucleic acids absorb UV light maximally at 280 nm.
In the light of the above statements, choose the most appropriate answer from the options given below :

This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
(A) is correct but (R) is not correct

Assertion (A) Analysis: UV Spectrophotometry for Nucleic Acids

Assertion (A) states that UV spectrophotometry is commonly used to estimate nucleic acids. This is correct because nucleic acids, like DNA and RNA, contain aromatic bases that absorb ultraviolet light.

Reason (R) Analysis: Nucleic Acid Absorbance Wavelength

Reason (R) claims that nucleic acids absorb UV light maximally at 280 nm. This statement is incorrect. While UV spectrophotometry is used, the characteristic maximal absorbance wavelength for nucleic acids (DNA and RNA) is approximately 260 nm. The 280 nm wavelength is typically associated with the absorbance of proteins, specifically aromatic amino acids like tyrosine and tryptophan.

Conclusion

Based on the analysis:

  • Assertion (A) is correct.
  • Reason (R) is incorrect.

Therefore, the correct choice is that (A) is correct but (R) is not correct.

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