Question
Correctly arrange the below given Transcription Factors (TFs) as per their involvement in initiation of eukaryotic transcription
A. TFIIB
B. TFIID
C. TFIIE
D. TFIIF
E. TFIIH
Choose the correct answer from the options given below:
This question was previously asked in
CUET PG 2026 Agri-Business Management Question Paper (25-Mar-2026) (Shift 2)
The correct answer is
B, A, D, C and E
Transcription Factor Order in Eukaryotic Initiation
Eukaryotic transcription initiation requires the sequential assembly of general transcription factors (GTFs) and RNA Polymerase II at the promoter to form the pre-initiation complex (PIC).
Eukaryotic Transcription Factor Roles and Assembly Sequence
The question requires arranging the following transcription factors:
- A. TFIIB
- B. TFIID
- C. TFIIE
- D. TFIIF
- E. TFIIH
The assembly order is determined by their specific functions:
- B (TFIID): This factor binds first to the promoter, typically recognizing the TATA box through its TATA-binding protein (TBP) subunit.
- A (TFIIB): TFIIB is recruited next. It helps stabilize TFIID binding and plays a role in selecting the transcription start site and bridging TFIID to RNA Polymerase II.
- D (TFIIF): TFIIF associates with RNA Polymerase II and assists in its recruitment to the promoter complex formed by TFIID and TFIIB.
- C (TFIIE): TFIIE binds subsequently and acts as a scaffold, primarily by recruiting TFIIH.
- E (TFIIH): As the final GTF in this sequence, TFIIH possesses crucial helicase and kinase activities. It unwinds the DNA at the start site (promoter melting) and phosphorylates the C-terminal domain (CTD) of RNA Polymerase II, enabling promoter escape and the start of elongation.
Based on this sequential functional requirement, the order is B, A, D, C, E.
Correct Transcription Factor Initiation Sequence
The progression of GTF involvement in eukaryotic transcription initiation follows this order:
B (TFIID) > A (TFIIB) > D (TFIIF) > C (TFIIE) > E (TFIIH)
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