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Question

Arrange the given steps of DNA fingerprinting in the sequence from initiation to end:

(A) Digestion of DNA by restriction endonuclease

(B) Isolation of DNA

(C) Hybridisation using labelled VNTR probe

(D) Transferring (blotting) of separated DNA fragments to synthetic membrane

The correct answer is

(B), (A), (D), (C)

The correct sequence for DNA fingerprinting is:

  • (B) Isolation of DNA from the sample.
  • (A) Digestion of DNA using restriction endonucleases to generate fragments.
  • (D) Transferring (blotting) of DNA fragments to a synthetic membrane like nylon.
  • (C) Hybridisation using labelled VNTR (Variable Number Tandem Repeats) probes to visualize specific sequences.
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Similar Questions

  1. Arrange the following steps of DNA fingerprinting in proper sequence:

    (A) Hybridisation using labelled VNTR probe

    (B) Separation of DNA fragments by electrophoresis

    (C) Digestion of DNA by restriction endonucleases

    (D) Blotting of separated DNA fragments to nylon

    (E) Isolation of DNA

    Choose the correct answer from the options given below:

  2. Nucleosome is:

  3. “Transforming Principle” was given by:

  4. Select the incorrect statement:

  5. Match List-I with List-II:

    List-I (Genes)List-II (Proteins – codes for lac operon)
    (A) ‘i’(I) permease
    (B) ‘a’(II) β-galactosidase
    (C) ‘y’(III) transacetylase
    (D) ‘z’(IV) repressor

    Choose the correct answer from the options given below:

  6. Amino acid is attached to which site of tRNA?

  7. Given below is the DNA coding sequence. Its complementary strand would read as:

    5′ – GTATTACG – 3′

  8. The size of VNTR varies from:

  9. In which phase of the cell cycle does replication of DNA take place?

  10. In Hershey and Chase experiment, some viruses grew on medium that contained:


Important Questions from Molecular Basis of Inheritance

  1. What will be the chromosome number in the gamete of fruit fly if its meiocyte has 8 chromosomes?

  2. Match List-I with List-II:

    List-I (Organism)List-II (Sex Chromosomes)
    (A) Male grasshopper(I) XY
    (B) Male Drosophila(II) XX 
    (C) Female bird(III) XX
    (D) Female grasshopper(IV) XO

    Choose the correct answer from the options given below:

  3. Match List-I with List-II:

    List-IList-II
    (A) Bacteriophage lambda(I) 231 gene
    (B) Y-chromosome of human(II) 48502 bp
    (C) Haploid content of human DNA (III) 3.3 × 109 bp
    (D) Escherichia coli DNA(IV) 4.6 × 106 bp

    Choose the correct answer from the options given below:

  4. Central dogma in molecular biology states that genetic information flows from:

  5. Read the following and select the set of correct statements. (A) Euchromatin is transcriptionally inactive (B) Heterochromatin is more densely packed (C) Heterochromatin is loosely packed (D) Euchromatin is transcriptionally active (E) Euchromatin stains lighter

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