Arrange the given steps of DNA fingerprinting in the sequence from initiation to end: (A) Digestion of DNA by restriction endonuclease (B) Isolation of DNA (C) Hybridisation using labelled VNTR probe (D) Transferring (blotting) of separated DNA fragments to synthetic membrane
(B), (A), (D), (C)
The correct sequence for DNA fingerprinting is:
- (B) Isolation of DNA from the sample.
- (A) Digestion of DNA using restriction endonucleases to generate fragments.
- (D) Transferring (blotting) of DNA fragments to a synthetic membrane like nylon.
- (C) Hybridisation using labelled VNTR (Variable Number Tandem Repeats) probes to visualize specific sequences.
Similar Questions
Which of the following statements are incorrect with respect to nucleotides?
(A) Purines and pyrimidines are nitrogenous bases.
(B) Nucleotides are non-enzymatic molecules.
(C) Phosphate group is linked to –OH of 5' C of a nucleoside through phosphoester linkage.
(D) In RNA, every nucleotide residue has an additional –OH group present at 2' position in the ribose.
(E) Thymine is an example of pyrimidine.
Nucleosome is associated with _______ molecules of histones.
Select the incorrect statement:
Match List-I with List-II:
List-I (Genes) List-II (Proteins – codes for lac operon) (A) ‘i’ (I) permease (B) ‘a’ (II) β-galactosidase (C) ‘y’ (III) transacetylase (D) ‘z’ (IV) repressor Choose the correct answer from the options given below:
The size of VNTR varies from:
Match List-I with List-II:
List-I (Scientists)
List-II (Discovery)
List-I List-II (A) Sutton and Boveri (I) X-Body (B) Sturtevant (II) Chromosomal Theory of Inheritance (C) Henking (III) Transformation in bacteria (D) Griffith (IV) Genetic maps Choose the correct answer from the options given below:
Select the observations drawn from the human genome project which are correct:
(A) The human genome contains 3164.7 million bp.
(B) The average gene consists of 3000 bases.
(C) Total number of genes is estimated at 30,000.
(D) The functions are unknown for over 50% of discovered genes.
(E) Less than 2% of the genome codes for proteins.
Arrange the following steps of DNA fingerprinting in proper sequence:
(A) Hybridisation using labelled VNTR probe
(B) Separation of DNA fragments by electrophoresis
(C) Digestion of DNA by restriction endonucleases
(D) Blotting of separated DNA fragments to nylon
(E) Isolation of DNA
Choose the correct answer from the options given below:
Nucleosome is:
“Transforming Principle” was given by:
Important Questions from Molecular Basis of Inheritance
Which of the following statements are incorrect with respect to nucleotides?
(A) Purines and pyrimidines are nitrogenous bases.
(B) Nucleotides are non-enzymatic molecules.
(C) Phosphate group is linked to –OH of 5' C of a nucleoside through phosphoester linkage.
(D) In RNA, every nucleotide residue has an additional –OH group present at 2' position in the ribose.
(E) Thymine is an example of pyrimidine.
Nucleosome is associated with _______ molecules of histones.
Two nucleotides linked together to form a dinucleotide through:
ABO blood groups are controlled by _______.
Match List-I with List-II:
List-I (Name of the organism) List-II (Chromosome number in a meiocyte) (A) Rat (I) 38 (B) Dog (II) 42 (C) Cat (III) 1260 (D) Ophioglossum (IV) 78 Choose the correct answer from the options given below:
