Gram Staining: Widely Used Bacteriology Technique
Gram staining is a fundamental and most widely employed microscopic staining method in bacteriology.
It is a differential stain, meaning it categorizes bacteria into two large groups based on their physical and chemical properties:
- Gram-positive bacteria (retain crystal violet stain)
- Gram-negative bacteria (counterstained with safranin)
This classification is crucial for the initial identification of microorganisms and guides subsequent diagnostic procedures and treatment strategies. Its effectiveness and simplicity make it a standard technique in virtually all microbiology laboratories worldwide.
While other staining methods exist (like Acid staining or specific stains like Safranin, which is part of the Gram procedure), none are as universally applied for initial bacterial characterization as Gram staining.
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Important Questions from Miscellaneous
- Which of the following relationships is/are not true?
(A). Most probable velocity = $\sqrt{\frac{2RT}{M}}$
(B). PV = $\frac{3}{2}kT$
(C). Compressibility factor Z = $\frac{pV}{nRT}$
(D). Average kinetic energy of gas = $\frac{1}{2}kT$
Choose the correct answer from the options given below - Match List-I with List-II
List-I List-II Electronic Configuration First Ionisation energy (kJ mol$^{-1}$) (A). ns$^2$ (I). 2100 (B). ns$^2$np$^1$ (II). 1400 (C). ns$^2$np$^3$ (III). 800 (D). ns$^2$np$^6$ (IV). 900
Choose the correct answer from the options given below: - The shielding constant of a 2p electron (calculated using Slater's rules) is
- Match List-I with List-II
List-I List-II Spectroscopy Property (A). Raman (I). Polarizability (B). FTIR (II). Dipole Moment (C). UV-Visible (III). Absorbance (D). NMR (IV). Spin
Choose the correct answer from the options given below: - The structure of protein comprises of:
(A). Primary structure of protein is associated with amino acids
(B). Secondary structure of protein is associated to peptides
(C). Tertiary structure of protein is associated with polypeptide chains
(D). Quaternary structure of protein is associated with polypeptide chains
Choose the correct answer from the options given below:
