The question asks to identify the substance and its quantity that releases 4 kilocalories (kcal) of energy.
Dietary energy is primarily provided by three macronutrients: proteins, carbohydrates, and fats. Each gram of these macronutrients provides a different amount of energy, measured in kilocalories (kcal).
Let's examine the energy released by the quantities mentioned in the options:
| Option | Substance / Quantity | Approximate Energy Released | Calculation |
| 1 | 4 grams of proteins | 16 kcal | $4 \text{ grams} \times 4 \text{ kcal/gram} = 16 \text{ kcal}$ |
| 2 | one gram of protein | 4 kcal | $1 \text{ gram} \times 4 \text{ kcal/gram} = 4 \text{ kcal}$ |
| 3 | one gram of sugar (carbohydrate) | 4 kcal | $1 \text{ gram} \times 4 \text{ kcal/gram} = 4 \text{ kcal}$ |
| 4 | 4 grams of fat | 36 kcal | $4 \text{ grams} \times 9 \text{ kcal/gram} = 36 \text{ kcal}$ |
Based on the standard energy values:
Therefore, the statement "4 kilo calories of energy is released by one gram of protein" is accurate based on typical nutritional values.