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Question

Which one of the following microbes causes acidification and curdling of milk?

This question was previously asked in
NDA I 2017 GAT Previous Year Paper (23-Apr-2017)
The correct answer is

Lactic Acid Bacillus

Understanding Milk Acidification and Curdling Microbes

Milk undergoes a process called acidification and curdling, primarily due to the action of specific types of microbes. This process is fundamental in the production of dairy products like yogurt and cheese.

The Role of Microbes in Milk Transformation

Milk contains a sugar called lactose. Certain microbes can metabolize lactose and convert it into lactic acid. The accumulation of lactic acid lowers the pH of the milk, making it acidic. This increased acidity causes the milk proteins (primarily casein) to coagulate or curdle, resulting in a thicker, semi-solid texture.

Identifying the Microbe Responsible for Milk Acidification and Curdling

We are looking for a microbe known to cause this specific transformation in milk.

  • Lactic Acid Bacillus: This term generally refers to rod-shaped bacteria that produce lactic acid as a major metabolic product of lactose fermentation. These are the key players in the traditional method of milk curdling. Genera like Lactobacillus fall under this category and are widely used in dairy fermentation. They consume lactose and produce lactic acid, leading to acidification and curdling.
  • Clostridium botulinum: This bacterium is known for producing botulinum toxin, a potent neurotoxin. It is associated with food poisoning, particularly in improperly canned foods, not milk curdling.
  • Vibrio cholera: This is a bacterium that causes cholera, a severe diarrheal disease. It is transmitted through contaminated water and food but is not involved in milk fermentation or curdling.
  • Saccharomyces cerevisiae: This is a type of yeast commonly used in baking (baker's yeast) and brewing (brewer's yeast). While yeasts are microbes, Saccharomyces cerevisiae ferments sugars into ethanol and carbon dioxide, not primarily lactic acid, and is not the main cause of milk curdling.

Based on the metabolic activity that leads to acidification and protein coagulation in milk, the microbes collectively referred to as Lactic Acid Bacillus (or Lactic Acid Bacteria) are responsible for this process.

Microbe Primary Action on Milk/Food Result
Lactic Acid Bacillus (Lactic Acid Bacteria) Ferments lactose into lactic acid Acidification and curdling of milk
Clostridium botulinum Produces botulinum toxin Food poisoning (not milk curdling)
Vibrio cholera Causes cholera Disease (not milk curdling)
Saccharomyces cerevisiae Ferments sugars into ethanol and CO<sub>2</sub> Used in baking/brewing (not primary cause of milk curdling)

Therefore, the microbe that causes acidification and curdling of milk is Lactic Acid Bacillus.

Revision Table: Key Microbes in Milk

Reviewing the roles of different microbes helps clarify their impact on milk.

  • Lactic Acid Bacteria: Essential for turning milk into yogurt, curd, cheese.
  • Pathogenic Bacteria (e.g., Clostridium botulinum, Vibrio cholera): Cause spoilage or disease, not desirable fermentation.
  • Yeasts (e.g., Saccharomyces cerevisiae): Primarily involved in alcoholic or CO<sub>2</sub> fermentation, not typical milk curdling.

Additional Information: The Science of Curdling

The curdling process by Lactic Acid Bacteria is a type of fermentation. The bacteria produce enzymes that break down lactose. The resulting lactic acid lowers the pH. At a pH of around 4.6, casein proteins in milk become unstable and clump together, forming the curd. This process not only preserves the milk longer (due to acidity) but also makes some nutrients more digestible and adds characteristic flavours and textures to dairy products.

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