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Question

Minifiler ™, the first commercial kit designed to amplify Mini STRs was developed by________

The correct answer is
Life Technologies

Minifiler Kit Developer Identified

The question asks to identify the developer of the Minifiler™ kit, recognized as the first commercial kit specifically designed for the amplification of Mini STRs (Short Tandem Repeats).

Mini STRs are smaller versions of STRs used in DNA profiling, particularly useful when DNA samples are degraded or limited.

The development of specialized kits like Minifiler™ was crucial for advancing DNA analysis techniques.

Developer of Minifiler™ Kit

The Minifiler™ kit, enabling the amplification of Mini STRs, was developed by Life Technologies.

Below are the provided options:

  • Applied Biosystems
  • Promega Corporation
  • Life Technologies
  • Qiagen

Therefore, Life Technologies is the correct answer.

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Important Questions from Molecular Biology Techniques

  1. Which one of the following does NOT use RNA-sequencing?

  2. Which one of the following statements related to molecular cloning procedures is INCORRECT?

  3. Amongst the following, which one is the most appropriate strategy to sequence and assemble highly repeated regions of a genome?

  4. Given below are statements related to various molecular techniques

    A. During molecular cloning of DNA fragments, a vector and insert molecule digested with two different enzymes can never be ligated with each other.

    B. Only 3' → 5' exonucleases and not 5' → 3' exonucleases can be used for digesting nucleic acids to generate blunt-ended fragments for cloning.

    C. In Sanger's dideoxy sequencing method, each reaction consists of a mixture of three dNTPs and one ddNTP.

    D. Self-ligation of a vector with compatible ends can be prevented by treatment with alkaline phosphatase.

    Which one of the following options represents a combination of correct statements?

  5. Given below are four DNA sequences and a set of forward and reverse primers for PCR amplification.

    SequencePrimes
    A 5' ACAATCGT.....ACTAGTAC-3'FP: 5'-TGTTAG-3'
    RP: 5'-TAGTAC-3'
    B5' AGTCTTAG.....ATGCCAGT - 3'FP: 5'-AAGACT-3'
    RP: 5'-ACTGGC-3'
    C5' CTTGACTA.....GTACAGTCA-3'FP: 5'-CTTGAC-3'
    RP: 5'-TGACTG-3'
    DD 5' GATCTAGC.....TCAAGCAGAC-3'FP: 5'-GATCTA-3'
    RP: 5'-CAGACG-3'
    In the absence of any other factors such as (but not restricted to) Tm, length, percent GC, etc., which one of the above template-primers combinations would produce an amplified fragment?
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