In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of 12.5 cm and the solvent front showed migration of 18 cm. The $R_f$ value for the compound is ______.
TLC Rf Value Calculation
The question asks for the retardation factor ($R_f$) value in a Thin Layer Chromatography (TLC) experiment.
The $R_f$ value is a measure used in chromatography to compare the movement of compounds. It is defined as the ratio of the distance traveled by the substance (spot) to the distance traveled by the solvent front.
Rf Value Formula
The formula for the $R_f$ value is:
$ R_f = \frac{\text{Distance traveled by the spot}}{\text{Distance traveled by the solvent front}} $
Given Data
- Distance traveled by the spot = 12.5 cm
- Distance traveled by the solvent front = 18 cm
Calculation
Substitute the given values into the formula:
$ R_f = \frac{12.5 \text{ cm}}{18 \text{ cm}} $
Calculating the value:
$ R_f \approx 0.694 $
Result Analysis
The calculated $R_f$ value is approximately 0.694. This value falls within the range specified (0.68 to 0.7), confirming the correct calculation for the compound's migration on the silica gel plate.
Important Questions from Methods in Microbiology
- Which one of the following programs is used for finding distantly related (or remote) protein homologs?
- Which one of the following is used for global alignment of two protein sequences?
- Correctly match the following Bioinformatic tool/Database with its respective Utility.
Bioinformatic tool/Database Utility P. BLAST 1. Database for 3D protein structures Q. Bowtie 2. Tool to identify similarity of a query sequence to existing sequences available in databanks R. AlphaFold 3. Tool to align short read DNA sequences obtained from Next-generation sequencing to a reference genome S. PDB 4. AI tool to predict protein structures - Which of the following is NOT used for generating an optimal alignment of two nucleotide sequences?
- Which one of the following BLAST search programs is used to identify homologs of a genomic DNA query in a protein sequence database?
