Bioinformatic tool/Database Utility P. BLAST 1. Database for 3D protein structures Q. Bowtie 2. Tool to identify similarity of a query sequence to existing sequences available in databanks R. AlphaFold 3. Tool to align short read DNA sequences obtained from Next-generation sequencing to a reference genome S. PDB 4. AI tool to predict protein structures
Bioinformatics Tool Identification
This question requires matching specific bioinformatics tools and databases with their primary functions.
- P. BLAST: Used to search for sequence similarity between a query sequence and sequences in a database. This corresponds to Utility 2.
- Q. Bowtie2: A fast and memory-efficient tool for aligning short DNA sequences (reads) from next-generation sequencing (NGS) to a reference genome. This corresponds to Utility 3.
- R. AlphaFold: An AI system designed to predict the 3D structure of proteins with high accuracy. This corresponds to Utility 4.
- S. PDB (Protein Data Bank): A widely used database containing experimental 3D structural data of large biological molecules, primarily proteins. This corresponds to Utility 1.
Correct Utility Matching
Based on the identification above, the correct matches are:
- P matches with Utility 2 (BLAST - sequence similarity search).
- Q matches with Utility 3 (Bowtie2 - short read alignment).
- R matches with Utility 4 (AlphaFold - AI protein structure prediction).
- S matches with Utility 1 (PDB - database for 3D protein structures).
Final Answer Verification
The combination P-2, Q-3, R-4, S-1 represents the correct pairing of tools/databases with their utilities. This corresponds to Option B.
Important Questions from Methods in Microbiology
To determine the viable cell count of a bacterial culture, you have plated $50 \mu \text{L}$ of a 100-fold diluted sample of the culture on a nutrient agar plate and obtained 20 colonies after overnight incubation. The viable cell count of the culture is ______ $\text{CFU mL}^{-1}$. (answer in integer)
- Which one of the following organisms is used for the determination of phenol coefficient of a disinfectant?
- Which one of the following programs is used for finding distantly related (or remote) protein homologs?
- Which one of the following is used for global alignment of two protein sequences?
- Which one of the following BLAST search programs is used to identify homologs of a genomic DNA query in a protein sequence database?
