Given below are two sets of terms related to various methods used in biological science. Which one of the following options correctly matches terms of Column A and Column B?Column A Column B A. RACE (i) DNA-protein interactions B. South-Western blotting (ii) FAM C. Recursive PCR (iii) Determining
the ends of mRNAD. TaqMan (iv) Construction
of synthetic DNA
Understanding Biological Methods and Their Applications
The question asks us to match different biological techniques listed in Column A with their specific applications or associated terms listed in Column B. Let's analyze each term in Column A and find its correct match in Column B.
Analyzing Column A and Column B Terms
- RACE (Rapid Amplification of cDNA Ends): This is a molecular biology technique used to obtain the full-length sequence of an RNA transcript. Specifically, it's used to determine the sequence of the 5' and 3' ends of messenger RNA (mRNA) that are not fully included in the initially cloned cDNA. Therefore, RACE is directly related to "Determining the ends of mRNA". This matches option (iii) in Column B.
- South-Western blotting: This technique is used to detect and characterize proteins that bind to specific DNA sequences. It is a method for studying DNA-protein interactions. A sample containing proteins is run on an electrophoresis gel, transferred to a membrane, and then probed with a labeled DNA sequence to see which proteins bind to it. This matches option (i) in Column B.
- Recursive PCR: This refers to a method for synthesizing large DNA molecules, such as entire genes, by assembling smaller overlapping oligonucleotide fragments in a recursive process involving PCR. It is used in the artificial construction of DNA sequences. This matches option (iv) in Column B.
- TaqMan: This is a brand name for a type of probe-based quantitative real-time PCR (qPCR) technique. TaqMan probes are oligonucleotides labeled with a fluorescent reporter dye (like FAM or VIC) and a quencher dye. During PCR amplification, the reporter dye is cleaved from the quencher, leading to an increase in fluorescence signal proportional to the amount of amplified DNA. FAM is a commonly used fluorescent dye in TaqMan probes. This matches option (ii) in Column B.
Matching the Columns
Based on the analysis above, we can establish the following matches:
- A. RACE → (iii) Determining the ends of mRNA
- B. South-Western blotting → (i) DNA-protein interactions
- C. Recursive PCR → (iv) Construction of synthetic DNA
- D. TaqMan → (ii) FAM
Let's represent this matching in a table format for clarity.
| Column A (Method) | Column B (Application/Term) | Match |
|---|---|---|
| A. RACE | (iii) Determining the ends of mRNA | A - (iii) |
| B. South-Western blotting | (i) DNA-protein interactions | B - (i) |
| C. Recursive PCR | (iv) Construction of synthetic DNA | C - (iv) |
| D. TaqMan | (ii) FAM | D - (ii) |
Final Match
The correct combination of matches is A - (iii), B - (i), C - (iv), and D - (ii).
Important Questions from Molecular Biology Techniques
Which one of the following does NOT use RNA-sequencing?
Which one of the following statements related to molecular cloning procedures is INCORRECT?
Amongst the following, which one is the most appropriate strategy to sequence and assemble highly repeated regions of a genome?
Given below are statements related to various molecular techniques
A. During molecular cloning of DNA fragments, a vector and insert molecule digested with two different enzymes can never be ligated with each other.
B. Only 3' → 5' exonucleases and not 5' → 3' exonucleases can be used for digesting nucleic acids to generate blunt-ended fragments for cloning.
C. In Sanger's dideoxy sequencing method, each reaction consists of a mixture of three dNTPs and one ddNTP.
D. Self-ligation of a vector with compatible ends can be prevented by treatment with alkaline phosphatase.
Which one of the following options represents a combination of correct statements?
Given below are four DNA sequences and a set of forward and reverse primers for PCR amplification.
In the absence of any other factors such as (but not restricted to) Tm, length, percent GC, etc., which one of the above template-primers combinations would produce an amplified fragment?Sequence Primes A 5' ACAATCGT.....ACTAGTAC-3' FP: 5'-TGTTAG-3'
RP: 5'-TAGTAC-3'B 5' AGTCTTAG.....ATGCCAGT - 3' FP: 5'-AAGACT-3'
RP: 5'-ACTGGC-3'C 5' CTTGACTA.....GTACAGTCA-3' FP: 5'-CTTGAC-3'
RP: 5'-TGACTG-3'D D 5' GATCTAGC.....TCAAGCAGAC-3' FP: 5'-GATCTA-3'
RP: 5'-CAGACG-3'
