A. Declaration of European core loci
B. Launching of Y-STR kit
C. Launching of first STR kit (CTT)
D. Launching of X-STR kit.
Choose the correct answer from the options given below:
DNA Profiling Events: Chronological Order
Understanding the timeline of significant developments in DNA profiling is crucial for exam preparation. This solution arranges the given landmark events in their historical sequence.
Sequence of Landmark DNA Profiling Events
The chronological order is determined by the approximate timing of each event's occurrence or widespread introduction in the field of forensic science:
- C. Launching of first STR kit (CTT): This represents the initial commercialization of autosomal Short Tandem Repeat (STR) multiplex systems. (Approx. 1997)
- A. Declaration of European core loci: This involved establishing a standardized set of STR loci for forensic databases across Europe to improve interoperability. (Approx. early 2000s)
- B. Launching of Y-STR kit: This refers to the introduction of kits specifically targeting the Y chromosome for genetic analysis. (Approx. mid-2000s)
- D. Launching of X-STR kit: This marks the availability of kits focused on analyzing the X chromosome. (Approx. mid-2000s onwards)
Determining the Correct Order
By ordering these events based on their historical progression, we establish the sequence:
C → A → B → D
This sequence reflects the advancement from early autosomal STR multiplexes to standardization efforts, followed by specialized Y-STR and X-STR systems.
Important Questions from Molecular Biology Techniques
Which one of the following does NOT use RNA-sequencing?
Which one of the following statements related to molecular cloning procedures is INCORRECT?
Amongst the following, which one is the most appropriate strategy to sequence and assemble highly repeated regions of a genome?
Given below are statements related to various molecular techniques
A. During molecular cloning of DNA fragments, a vector and insert molecule digested with two different enzymes can never be ligated with each other.
B. Only 3' → 5' exonucleases and not 5' → 3' exonucleases can be used for digesting nucleic acids to generate blunt-ended fragments for cloning.
C. In Sanger's dideoxy sequencing method, each reaction consists of a mixture of three dNTPs and one ddNTP.
D. Self-ligation of a vector with compatible ends can be prevented by treatment with alkaline phosphatase.
Which one of the following options represents a combination of correct statements?
Given below are four DNA sequences and a set of forward and reverse primers for PCR amplification.
In the absence of any other factors such as (but not restricted to) Tm, length, percent GC, etc., which one of the above template-primers combinations would produce an amplified fragment?Sequence Primes A 5' ACAATCGT.....ACTAGTAC-3' FP: 5'-TGTTAG-3'
RP: 5'-TAGTAC-3'B 5' AGTCTTAG.....ATGCCAGT - 3' FP: 5'-AAGACT-3'
RP: 5'-ACTGGC-3'C 5' CTTGACTA.....GTACAGTCA-3' FP: 5'-CTTGAC-3'
RP: 5'-TGACTG-3'D D 5' GATCTAGC.....TCAAGCAGAC-3' FP: 5'-GATCTA-3'
RP: 5'-CAGACG-3'
