Which technique identifies a specific DNA sequence using a radiolabelled DNA probe ?
Southern Blotting
The blotting techniques are named for the molecule they detect, and the one that detects DNA with a labelled DNA probe is Southern blotting, devised by Edwin Southern in 1975.
The steps are: digest the DNA with restriction enzymes, separate the fragments by size on an agarose gel, denature them into single strands, transfer them onto a nitrocellulose or nylon membrane, and then hybridise with a radiolabelled complementary probe. Autoradiography shows a band exactly where the probe has bound, identifying the sequence sought.
The related methods target other molecules. Northern blotting detects RNA, Western blotting detects proteins using antibodies rather than nucleic-acid probes, and Eastern blotting is used for post-translational modifications of proteins.
Hence, the answer is Southern Blotting.
Important Questions from Biotechnology
Select wrong statement:
Mahabeej Biotechnology Center, Nagpur's state-of-art tissue culture laboratory produces ________ on large scale.
_________ is known as Biological containment.
The first vaccine developed from minced chick tissue against _________ found enormous application in tropical countries.
_________ can be sometimes used to determine percentage paternity of a child.
