Question
Shoot organogenesis by tissue culture results into
The correct answer is
a bipolar structure that has a strong connection with the pre-existing vascular tissue of the explant
Shoot Organogenesis Outcome in Tissue Culture
Shoot organogenesis is a method in plant tissue culture where new shoots develop directly from an explant (a piece of plant tissue).
Structure Characteristics
- Bipolar Development: The process typically involves the formation of organized structures that have the potential for both shoot and root development, characteristic of a bipolar organization, even if only the shoot apex is initially prominent.
- Vascular Integration: For the newly formed shoot to survive and grow, it must establish a functional vascular connection with the explant's existing vascular system. This ensures the transport of water and nutrients.
Conclusion on Vascular Connection
A successful shoot generated through organogenesis demonstrates a strong vascular connection with the pre-existing vascular tissue of the explant. This connection is vital for its sustenance and further development.
Evaluating Options
- Options 1 and 3 are incorrect because they state "no vascular connection," which is biologically unviable for sustained growth.
- Option 2 describes a "monopolar structure," which doesn't fully capture the organized developmental potential or the necessary vascular link inherent in successful organogenesis.
- Option 4 correctly identifies the outcome as a "bipolar structure" with a "strong connection" to the explant's vascular tissue.
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Important Questions from Totipotency and Plant Regeneration
- Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion [a]: A zygote and its immediate descendant cells are unspecialized and are called totipotent
Reason [r]: Totipotent cells retain the capacity to differentiate into only a few cell types - Determine the correctness or otherwise of the following Assertion [a] and the Reason [r]
Assertion [a]: It is possible to regenerate a whole plant from a single plant cell.
Reason [r]: It is easier to introduce transgenes in to plants than animals. - A dioecious plant has XX sexual genotype for female and XY for male. After double fertilization, what would be the genotype of the embryos and endosperms?
Determine the correctness or otherwise of the following Assertion and Reason (r).
Assertion: In direct somatic embryogenesis, embryos are developed without going through callus formation.
Reason: This is possible due to the presence of pre-embryonically determined cells.
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