Match List - I with List - II: Choose the correct answer from the options given below:List - I List - II (A) Structural genes in eukaryotes (I) Polycistronic (B) The coding sequence that appears in mature RNA (II) Monocistronic (C) Structural genes in Prokaryotes (III) Intron (D) Sequences not present in processed RNA (IV) Exons
A-(II), B-(IV), C-(I), D-(III)
(A) Structural genes in eukaryotes are typically (II) Monocistronic. This means that one mRNA molecule codes for only one protein.
(B) The coding sequence that appears in mature RNA are (IV) Exons. Exons are the parts of a gene that are present in the final, processed mRNA molecule and contain the protein-coding sequence.
(C) Structural genes in Prokaryotes are often (I) Polycistronic. This means that one mRNA molecule can code for multiple proteins that are functionally related and often organized in operons.
(D) Sequences not present in processed RNA are (III) Introns. Introns are non-coding sequences within a gene that are transcribed into pre-mRNA but are subsequently removed by RNA splicing during the processing of mRNA.
Therefore, the correct matches are:
- A - II
- B - IV
- C - I
- D - III
Important Questions from Biotechnology : Principles and Processes
A thermostable DNA polymerase is isolated from:
Which one of the following is not a process of DNA recombinant technology?
Downstream processing method involves:
Arrange the following steps involved in the transformation of bacteria in a sequence from initiation to end:
(A) Incubation of rDNA with bacterial cell on ice
(B) Treatment with divalent cations
(C) Heat shock treatment
(D) Selection on antibiotic-containing agar plate
(E) Placed them again on ice
Which of the following statements are incorrect?
(A) Fragments of DNA can be separated by ELISA.
(B) Transformation is a procedure through which a piece of DNA is introduced into a host bacterium.
(C) Recombinant DNA technology does not involve isolation of a desired DNA fragment.
(D) DNA ligases are used for stitching DNA fragments into a vector.
