Match List - I with List - II: Choose the correct answer from the options given below:List - I List - II (A) Structural genes in eukaryotes (I) Polycistronic (B) The coding sequence that appears in mature RNA (II) Monocistronic (C) Structural genes in Prokaryotes (III) Intron (D) Sequences not present in processed RNA (IV) Exons
A-(II), B-(IV), C-(I), D-(III)
(A) Structural genes in eukaryotes are typically (II) Monocistronic. This means that one mRNA molecule codes for only one protein.
(B) The coding sequence that appears in mature RNA are (IV) Exons. Exons are the parts of a gene that are present in the final, processed mRNA molecule and contain the protein-coding sequence.
(C) Structural genes in Prokaryotes are often (I) Polycistronic. This means that one mRNA molecule can code for multiple proteins that are functionally related and often organized in operons.
(D) Sequences not present in processed RNA are (III) Introns. Introns are non-coding sequences within a gene that are transcribed into pre-mRNA but are subsequently removed by RNA splicing during the processing of mRNA.
Therefore, the correct matches are:
- A - II
- B - IV
- C - I
- D - III
Important Questions from Biotechnology : Principles and Processes
The first restriction endonuclease to be isolated was:
Arrange the following steps of PCR in correct sequence:
- (A) Denaturation of ds DNA
- (B) Annealing
- (C) Amplification
- (D) Extension of Primer
- (E) Use of DNA polymerase and deoxynucleotides
Choose the correct answer from the options given below:
The process of cutting out DNA fragments from agarose gel and their extraction from gel piece is known as:
Arrange the following steps of rDNA technology in correct sequence.
(A) Amplification of gene by PCR
(B) Insertion of rDNA into host cell using vector
(C) Isolation of the genetic material from the cell
(D) Cutting the DNA at specific location
In biolistic method, the cells are bombarded with high velocity microparticles of:
