pBR322 BamHI Site and Antibiotic Resistance
The cloning vector pBR322 is a common plasmid used in genetic engineering, particularly in E. coli.
Antibiotic Resistance Genes in pBR322
pBR322 contains genes that provide resistance to two antibiotics:
- Ampicillin (resistance conferred by the amp gene)
- Tetracycline (resistance conferred by the tet gene)
BamHI Restriction Site Location
The recognition site for the restriction enzyme BamHI is located within the tetracycline resistance gene (tet gene) of the pBR322 vector.
Consequence of Foreign DNA Insertion
Inserting foreign DNA into a restriction site within a functional gene inactivates that gene.
Therefore, inserting foreign DNA at the BamHI site disrupts the tetracycline resistance gene, rendering it non-functional.
Impact on Antibiotic Resistance
As a result of the inactivation of the tetracycline resistance gene, bacteria transformed with this recombinant plasmid will lose their resistance to Tetracycline. The ampicillin resistance gene is typically unaffected by insertion at the BamHI site, so resistance to ampicillin is usually retained.
Similar Questions
- Which of the following statements are not true regarding restriction endonucleases ?
A. They are called molecular scissors.
B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
C. They cut the DNA only at the centre of the palindromic sites.
D. They remove nucleotides only from the ends of DNA fragments.
E. They recognise specific palindromic base-pair sequences.
Choose the correct answer from the options given below : - Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
- Which of the following statements are correct with respect to DNA separation, isolation and visualization ?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.
C. The separated DNA fragments can be seen without staining when exposed to UV light.
D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.
Choose the correct answer from the options given below : - Match List I with List II :
List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aquaticus D. pBR322 IV. Escherichia coli
Choose the correct answer from the options given below :
Important Questions from Biotechnology
- Which of the following statements are not true regarding restriction endonucleases ?
A. They are called molecular scissors.
B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
C. They cut the DNA only at the centre of the palindromic sites.
D. They remove nucleotides only from the ends of DNA fragments.
E. They recognise specific palindromic base-pair sequences.
Choose the correct answer from the options given below : - Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
- Which of the following statements are correct with respect to DNA separation, isolation and visualization ?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.
C. The separated DNA fragments can be seen without staining when exposed to UV light.
D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.
Choose the correct answer from the options given below : - Match List I with List II :
List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aquaticus D. pBR322 IV. Escherichia coli
Choose the correct answer from the options given below :
