Microscope Filter Identification
The question asks to identify the type of microscope that utilizes an excitation filter, a dichroic mirror, and barrier filters.
Filter Functions in Microscopy
These optical components are specifically designed to manage light wavelengths:
- Excitation Filter: Selects specific wavelengths from the light source to excite the fluorescent specimen.
- Dichroic Mirror (or Beamsplitter): Reflects the excitation light towards the specimen and transmits the longer-wavelength emission light from the specimen towards the detector.
- Barrier Filter (or Emission Filter): Blocks any remaining excitation light and transmits only the emitted fluorescence light to the observer or camera.
Microscope Type Correlation
The combination and function of these filters are defining characteristics of a Fluorescence microscope. This setup enables the selective visualization of fluorescently labeled structures within a sample by efficiently separating the excitation light from the emitted fluorescence.
Other microscope types use different principles:
- Polarizing microscopes use polarizing filters.
- Scanning electron microscopes use electron beams, not optical filters in this manner.
- Differential Interference Contrast (DIC) microscopes manipulate light interference patterns using specialized prisms and polarizers.
Therefore, the presence of excitation filters, dichroic mirrors, and barrier filters points directly to a fluorescence microscope.
Important Questions from Microscopic techniques
In electron microscopy, to detect specific macromolecule or structure such as spindle pole body (SPB), the frequently used procedure is to couple secondary antibody with
Which of the following components is typically NOT utilized while capturing images using a confocal laser scanning microscope (CLSM)?
The following statements were made about Laser Scanning Confocal Microscopy (LSCM).
A. LSCM is a wide field technique with Köhler illumination system.
B. Spatial resolution higher than that achieved in wide field imaging could be obtained if only the central portion of an Airy Disk is used to form an image.
C. Scanning mirrors sweep the excitation beam over the sample point-by-point to build the image.
D. An altered pinhole size does not make any impact on the resolution of the image.
E. A photomultiplier tube (PMT) in LSCM helps in generating real colour of fluorophores.
Which one of the following options represents the combination of all correct statements?
Arrange the following phases of microbial growth curve in correct sequence :
(A) Logarithmic or exponential phase
(B) Negative acceleration
(C) Lag phase
(D) Positive acceleration
(E) Maximal stationary phase
Choose the correct answer from the options given below :
- A side-by-side view of two specimens can be best obtained by the:________
