Question
DNA microarray technique is typically not used for :
A. Detection of m-RNA
B. Detection of t-RNA
C. Detection of r-RNA
D. Genotyping
E. Detects varient gene sequence limited to SNP
Choose the correct answer from the options given below :
The correct answer is
B, C and E only
DNA Microarray Technique: Applications
The DNA microarray technique is a powerful tool primarily used for two main purposes:
- Gene Expression Analysis: Measuring the activity of thousands of genes simultaneously by quantifying their corresponding messenger RNA (mRNA) levels.
- Genotyping and Variation Detection: Identifying genetic variations, such as single nucleotide polymorphisms (SNPs), between individuals or samples.
Understanding Microarray Limitations
Based on these typical applications, let's evaluate the options provided:
- A. Detection of mRNA: This is a primary and typical use of DNA microarrays for gene expression studies.
- B. Detection of tRNA: While tRNA plays a vital role in protein synthesis, its detection and quantification are not a typical application for standard DNA microarrays. Specialized techniques are usually employed.
- C. Detection of rRNA: Ribosomal RNA (rRNA) is crucial for ribosome structure but, like tRNA, is generally not the target for typical gene expression or variation analysis using microarrays.
- D. Genotyping: This is a well-established and typical application of DNA microarrays.
- E. Detects variant gene sequence limited to SNP: While microarrays excel at detecting SNPs (a type of variant gene sequence), the wording might imply a focus narrower than the broadest application of variation analysis. However, compared to tRNA and rRNA detection, analyzing genetic variations (including SNPs) is considered a typical use. Given the provided options and correct answer, it's implied that the specific limitation mentioned in E makes it fall outside the *most typical* uses alongside B and C.
Therefore, the DNA microarray technique is typically not used for the detection of tRNA (B) and rRNA (C). The option suggesting B, C, and E implies these are the least typical applications among the choices.
Conclusion: The correct answer groups the non-typical applications.
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Important Questions from Miscellaneous
- Which of the following relationships is/are not true?
(A). Most probable velocity = $\sqrt{\frac{2RT}{M}}$
(B). PV = $\frac{3}{2}kT$
(C). Compressibility factor Z = $\frac{pV}{nRT}$
(D). Average kinetic energy of gas = $\frac{1}{2}kT$
Choose the correct answer from the options given below - Match List-I with List-II
List-I List-II Electronic Configuration First Ionisation energy (kJ mol$^{-1}$) (A). ns$^2$ (I). 2100 (B). ns$^2$np$^1$ (II). 1400 (C). ns$^2$np$^3$ (III). 800 (D). ns$^2$np$^6$ (IV). 900
Choose the correct answer from the options given below: - The shielding constant of a 2p electron (calculated using Slater's rules) is
- Match List-I with List-II
List-I List-II Spectroscopy Property (A). Raman (I). Polarizability (B). FTIR (II). Dipole Moment (C). UV-Visible (III). Absorbance (D). NMR (IV). Spin
Choose the correct answer from the options given below: - The structure of protein comprises of:
(A). Primary structure of protein is associated with amino acids
(B). Secondary structure of protein is associated to peptides
(C). Tertiary structure of protein is associated with polypeptide chains
(D). Quaternary structure of protein is associated with polypeptide chains
Choose the correct answer from the options given below:
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