Consider the following stages of insulin production procedure through gene technology : 1. Isolation of insulin coding gene from humans 2. Inserting the insulin coding gene into a plasmid vector 3. Inserting the recombinant plasmid vector into the bacterium (E. coli) Which of the following is a correct sequence of the above?
1-2-3
Step 1: The gene coding for human insulin must first be isolated/identified from human DNA.
Step 2: This isolated gene is then inserted into a plasmid vector (such as from E. coli) using restriction enzymes and DNA ligase, creating a recombinant plasmid.
Step 3: The recombinant plasmid is finally introduced into the bacterium E. coli, which then expresses the human insulin gene and mass-produces insulin protein through fermentation, as pioneered commercially by products like Humulin.
Hence, the answer is 1-2-3.
Similar Questions
Which one of the following statements about natural selection is not correct?
Consider the following statements about genetic engineering :
1. The technique of genetic engineering involves creation of recombinant DNA, use of gene cloning and gene transfer.
2. Restriction enzymes cut DNA at specific locations.
3. Restriction enzymes split the entire double-stranded DNA into two strands.
Which of the statements given above is/are correct?
Which of the following is/are the basic step(s) in genetically modifying an organism?
1. Identification of DNA with desirable genes
2. Introduction of the identified DNA into the host
3. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny
Select the correct answer using the code given below.
Important Questions from Genetics and Evolution
Different varieties of the same gene are called
In a sexually reproducing organism, which one of the following statements is appropriate both for the parent and offspring?
In most prokaryotes, the chromosome number is:
Bacterial DNA is referred to as naked because it is not associated with:
The two important features of sexual reproduction in higher organisms that create genetic diversity in offspring are
